This behavior has changed several times since it was first introduced. In some cases, the original behavior was retained, in some cases, it has been improved and updated. Please see the instructions below for the specific versions.
Appearance and behavior in Version 2.0
In Version 2.0, the ROI icon will appear as shown above. To add multiple ROI's in your video you have to left-click, hold and drag the first box. It will be RED in color. To draw the second box, you had to press the Control [CTRL] key then left-click, hold and drag additional boxes. New boxes will be BLUE, GREEN, ORANGE, YELLOW, PINK, then RED with this sequence repeating. Between each ROI, you have to wait for the measurement calculations to complete before starting to position the next ROI.
Appearance and behavior in Version 2.1
In Version 2.1, the ROI Icons were changed to those shown above. In this version, a "Persist" mode was added.
When the Motion Amplification® software is set to "Standard Mode", your icon will look like this: 
. In this mode, you add multiple ROIs by following the same procedure as described above for Version 2.0.
When you click on the down arrow between the two icons, your icon will change to this: 
. To draw ROIs in this mode, you only need to left-click, hold and drag with your mouse. Every time you release, an ROI will appear. When you click another location, you will begin drawing a new ROI. Between each ROI, you have to wait for the measurement calculations to complete before starting to position the next ROI.
To switch between the two, simply click on the arrow to the right of the ROI icon, 
. Choose the mode you would like to activate and click on the name to close the menu:
Appearance and behavior in Version 2.2
In Version 2.2, Standard Mode was changed to "Independent Mode" as shown above. The behavior is still as described in Version 2.0 above, only the name was changed. "Persist Mode" behaves as described in the Version 2.1 section above.
Appearance and behavior in Version 2.3 and Version 2.4
In Version 2.3, the ROI Icon has returned to the original design. However, the behavior is as described under "Persist Mode" in Version 2.1 above. Simply left-click, hold and drag to add ROIs to the video. You can still hold the CTRL, left-click and hold to draw multiple ROIs as well.
You can now click the "Cancel" button as the first measurements are calculated. Early measurements will be suspended until all of the ROIs are placed. Allow the measurements to calculate after placing your LAST ROI and all previously placed ROIs will have their measurements calculated at once saving you time.
For example, if you want to place four ROIs in your video, you can left-click, hold and drag to place the first ROI. As the measurement dialog appears, click "Cancel" and place the second and third ROIs in this same manner. When you place the fourth ROI, allow the measurements to calculate and all four will be presented when done.
Appearance and behavior in Version 2.5
In Version 2.5, the order of the two ROI icons is reversed. Delete is on the left and the Type of ROI selection is on the right. In this mode, you place ROIs by left-clicking, hold and drag in the area where you want to ROI. You can place multiple ROIs by continuing this process everywhere you want an ROI. You can suspend the measurement calculations and complete them with the last ROI placed by clicking the "Cancel" button as the calculation window appears.
In this version, you have two new ROI types, the “Horizontal Shaft” and the “Vertical Shaft” measurement tools. As the icons indicate, you will be able to measure shaft movement on a shaft which is recorded horizontally using the Horizontal Shaft tool. Shafts recorded in a vertical orientation are measured using the Vertical Shaft tool.
Appearance and behavior in Version 3.0
The ROI Icons appearance is unchanged in Version 3.0, but there is a new “Transient” ROI tool.
This type of ROI will track translational movement and show you the path of such motion.
PURPLE was introduced as a 7th color in the sequence in this version. Now the color cycle, RED, BLUE, GREEN, ORANGE, YELLOW, PINK, PURPLE, RED, will repeat.
Appearance and behavior in Version 3.1
The left "Delete ROI" icon has been removed. The ROI Type selection list remains unchanged when clicking on the down arrow to the right of the ROI tool.
To delete an ROI, select it and then select "Delete/Delete All ROIs" from the pop-up menu.
Appearance and behavior in Version 3.5 - Delayed Measurement Calculations for individual ROIs
You can now place multiple ROIs at one time and delay calculating the measurements until after the last ROI is placed.
To place multiple ROIs press and hold the Control [CTRL] key on the keyboard. Then, left-click hold and drag to draw an ROI box. Release the left mouse button. The ROI will appear with a cross through the center. Continue to hold CRTL and draw the next box. Place as many ROIs as necessary. When placing the final ROI, first release the CTRL button, then click hold and drag to place the final ROI. When you release the left mouse button, all calculations will be performed.
Appearance and behavior in Version 3.7
Now, when placing multiple ROIs, the calculations begin as soon as the CTRL button is released.
New in Version 3.7 - Fixed Size ROIs
A new ROI type is added to the selection list. Selecting this option will allow you to click-hold and drag ROI boxes in the image. When you finish drawing the box, it will immediately resize to a specific size.
The first time you use this feature, the dialog below will appear. It explains where to locate the settings for the Fixed Size ROI box.
At the bottom of the "ROI Options" section of the Motion Amplification Options window, you can define the Width and Height you would like the Fixed Size ROI boxes to be.
You can place multiple Fixed Size ROIs by pressing and holding the Control [CTRL] key on the keyboard. Then, left-click hold and drag to draw an ROI box. Release the left mouse button. The ROI will appear with a cross through the center. Continue to hold CRTL and draw the next box. Place as many ROIs as necessary. After placing the final ROI, release the CTRL button and all calculations will be performed.
Pressing and holding the Alternate [ALT] key will cause a Fixed Size ROI to be placed every time you single click a location.
The above two actions, holding [CTRL] and [ALT] keys together, will change ROI placement to place a fixed size ROI every time you single click in the image. Releasing the two keys will cause all recently placed ROIs to perform their measurement calculations.
New in Version 3.7 - Duplicate ROIs
Select an ROI box. The "Edit Grab Handles" will be displayed. Once an ROI is active, right-click in the area. The new pop-up menu will show a new option to "Duplicate ROI/Duplicate All ROIs". Selecting Duplicate ROI will cause the software to place an identical ROI Type and Size near the original. If the ROI is named, the name of the new ROI will match the existing format. You can then drag the new ROI to the desired location.
Selecting "Duplicate All ROIs" will place a second identical ROI near EVERY ROI in view. Be careful! Duplicating lots of ROIs adds up in a hurry!!
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